One of the first techniques that come to mind when it comes to gene editing in the scientific world in recent years is CRISPR-Cas9. However, the negative aspects of the technique push the experts to develop new techniques.
Although gene editing methods bring a lot of controversy, scientific developments that have the potential to save lives. It will enable new technical researches to be done faster.
Retron Library Recombineering
Researchers at Harvard’s Wyss Institute have developed a new gene editing technique called Retron Library Recombineering (RLR). Single-stranded DNA fragments can be produced in the RLR, which uses bacteria ‘DNA segments called retrons.
On the other hand, in addition to the fact that the materials used in the CRISPR technique are difficult to obtain in large numbers, the Cas9 enzyme used can cut the parts outside the targeted DNA segments.
In CRISPR-Cas9, which stands out with higher accuracy than gene editing techniques until now, mutant sequences are added to the genome during cell division, while in RLR the mutant chain is added only to newly formed DNA.
Therefore, the main DNA sequence is not damaged, which paves the way for the original DNA to be subjected to a large number of experiments.
In addition, retron sequences also act as labels for scientists, making it easy to follow different samples in the bacterial pool.
Finally, we will have to wait until the RLR, which scientists think can lead to unexpected exciting innovations, becomes applicable in daily life.